Mechanistic studies of the long chain acyl-CoA synthetase Faa1p from Saccharomyces cerevisiae
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چکیده
منابع مشابه
Structure and regulation of rat long-chain acyl-CoA synthetase.
Complementary DNAs encoding rat long-chain acyl-CoA synthetase have been isolated. The cDNAs were identified using synthetic oligonucleotide probes based on partial amino acid sequences of lysyl endopeptidase peptides of the purified enzyme. Rat long-chain acyl-CoA synthetase is predicted to contain 699 amino acid residues and to have a calculated molecular weight of 78,177. Significant sequenc...
متن کاملAcyl-CoA Dehydrogenases: Mechanistic studies on Medium Chain Acyl-CoA Dehydrogenase
ion of Glu99, a group with potential base functionality that is also present at the “bottom” of the active center (Kim et al., 1993); b) to gain information on whether the activity observed with the Glu376Gln mutant is specific to the 376Gln amide group itself. The pH dependence of the enzyme flavin reduction rates for these mutants was measured as described above for Glu376Gln-MCAD under anaer...
متن کاملMechanistic studies on fatty acyl-CoA dehydrogenase.
It has previously been shown that the "partial" reaction between fatty acyl-CoA dehydrogenase and acyl-CoA substrate is pH-dependent (larger rate constants at basic pH) and shows a biphasic rate profile indicative of formation of an initial charge transfer complex between the C-2 anion of substrate and enzyme. The present investigation indicates that the complete reaction between acyl-CoA and e...
متن کاملThe Acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular Utilization.
Exogenous long-chain fatty acids are activated to coenzyme A derivatives prior to metabolic utilization. In the yeast Saccharomyces cerevisiae, the activation of these compounds prior to metabolic utilization proceeds through the fatty acyl-CoA synthetases Faa1p and Faa4p. Faa1p or Faa4p are essential for long-chain fatty acid import, suggesting that one or both of these enzymes are components ...
متن کاملA Peroxisomal Long-Chain Acyl-CoA Synthetase from Glycine max Involved in Lipid Degradation
Seed storage oil, in the form of triacylglycerol (TAG), is degraded to provide carbon and energy during germination and early seedling growth by the fatty acid β-oxidation in the peroxisome. Although the pathways for lipid degradation have been uncovered, understanding of the exact involved enzymes in soybean is still limited. Long-chain acyl-CoA synthetase (ACSL) is a critical enzyme that acti...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
سال: 2007
ISSN: 1388-1981
DOI: 10.1016/j.bbalip.2007.05.009